Genome editing in the human malaria parasite Plasmodium falciparum using the CRISPR-Cas9 system.
basic_science · Level V
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- Record sourced from PubMed, PMID 24880488.
- Also identified by DOI 10.1038/nbt.2925.
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Abstract
Genome manipulation in the malaria parasite Plasmodium falciparum remains largely intractable and improved genomic tools are needed to further understand pathogenesis and drug resistance. We demonstrated the CRISPR-Cas9 system for use in P. falciparum by disrupting chromosomal loci and generating marker-free, single-nucleotide substitutions with high efficiency. Additionally, an artemisinin-resistant strain was generated by introducing a previously implicated polymorphism, thus illustrating the value of efficient genome editing in malaria research.
Medical subject headings
- Clustered Regularly Interspaced Short Palindromic Repeats
- Genome, Protozoan
- Plasmodium falciparum