Mapping the calcification of bovine pericardium in rat model by enhanced micro-computed tomography.

Liu, Jing; Zhong, Shengping; Lan, Hualin; Meng, Xu; Zhang, Haibo; Fan, Yubo; Wang, Yuxing; Wang, Chunren et al. · Biomaterials · 2014

basic_science · Level V

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Abstract

The calcification initiation and progression of bioprosthetic heart valve were investigated in a rat model by enhanced micro-computed tomography, together with histologic study and scanning electron microscope analysis. The implantation data at early stage showed apparent dendritic patterns in the radiographic images for the glutaraldehyde-treated bovine pericardium and this dendritic pattern was verified to be associated with the vessel distribution in the tissue. Histologic study and scanning electron microscope analysis both indicated that the calcium deposits in the pericardium vessels regions were more grievous than those scattered in the collagen fibers in the first two weeks after implantation. Subsequently, calcification spreaded and the entire sample was severely calcified in 60 days.

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