What's changed with genome editing?
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 24996161.
- Also identified by DOI 10.1016/j.stem.2014.06.017.
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Abstract
Targeted genome editing using engineered nucleases can reliably generate defined genetic mutations, although the range of off-target lesions caused by these techniques is unclear. Recent papers in Cell Stem Cell perform high-coverage whole-genome sequencing to demonstrate the feasibility of obtaining correctly targeted pluripotent stem cell clones with minimal unintended modifications.
Medical subject headings
- Deoxyribonucleases
- High-Throughput Nucleotide Sequencing
- Pluripotent Stem Cells