Microfluidic cryofixation for correlative microscopy.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 25007311.
- Also identified by DOI 10.1039/c4lc00333k.
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Abstract
Cryofixation yields outstanding ultrastructural preservation of cells for electron microscopy, but current methods disrupt live cell imaging. Here we demonstrate a microfluidic approach that enables cryofixation to be performed directly in the light microscope with millisecond time resolution and at atmospheric pressure. This will provide a link between imaging/stimulation of live cells and post-fixation optical, electron, or X-ray microscopy.
Medical subject headings
- Cold Temperature
- Microfluidics
- Microscopy