GlycoDigest: a tool for the targeted use of exoglycosidase digestions in glycan structure determination.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 25015990.
- Also identified by DOI 10.1093/bioinformatics/btu425 and PMC identifier 4609004.
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Abstract
Sequencing oligosaccharides by exoglycosidases, either sequentially or in an array format, is a powerful tool to unambiguously determine the structure of complex N- and O-link glycans. Here, we introduce GlycoDigest, a tool that simulates exoglycosidase digestion, based on controlled rules acquired from expert knowledge and experimental evidence available in GlycoBase. The tool allows the targeted design of glycosidase enzyme mixtures by allowing researchers to model the action of exoglycosidases, thereby validating and improving the efficiency and accuracy of glycan analysis. http://www.glycodigest.org.
Medical subject headings
- Glycoside Hydrolases
- Oligosaccharides
- Software