Spatiotemporal intracellular nitric oxide signaling captured using internalized, near-infrared fluorescent carbon nanotube nanosensors.
basic_science · Level V
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- Record sourced from PubMed, PMID 25029087.
- Also identified by DOI 10.1021/nl502338y and PMC identifier 4134139.
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Abstract
Fluorescent nanosensor probes have suffered from limited molecular recognition and a dearth of strategies for spatial-temporal operation in cell culture. In this work, we spatially imaged the dynamics of nitric oxide (NO) signaling, important in numerous pathologies and physiological functions, using intracellular near-infrared fluorescent single-walled carbon nanotubes. The observed spatial-temporal NO signaling gradients clarify and refine the existing paradigm of NO signaling based on averaged local concentrations. This work enables the study of transient intracellular phenomena associated with signaling and therapeutics.
Medical subject headings
- Fluorescence
- Human Umbilical Vein Endothelial Cells
- Nanotubes, Carbon
- Nitric Oxide
- Signal Transduction