Optimized cell transplantation using adult rag2 mutant zebrafish.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 25042784.
- Also identified by DOI 10.1038/nmeth.3031 and PMC identifier 4294527.
- No licence information is recorded for this record.
- Because redistribution is not established, this page shows the abstract only. Follow the links below for the full text.
Abstract
Cell transplantation into adult zebrafish has lagged behind mouse models owing to the lack of immunocompromised strains. Here we have created rag2(E450fs) mutant zebrafish that have reduced numbers of functional T and B cells but are viable and fecund. Mutant fish engraft muscle, blood stem cells and various cancers. rag2(E450fs) mutant zebrafish are the first immunocompromised zebrafish model that permits robust, long-term engraftment of multiple tissues and cancer.
Medical subject headings
- Cell Transplantation
- DNA-Binding Proteins
- Mutation
- Zebrafish