Single-cell genome-wide bisulfite sequencing for assessing epigenetic heterogeneity.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 25042786.
- Also identified by DOI 10.1038/nmeth.3035 and PMC identifier 4117646.
- No licence information is recorded for this record.
- Because redistribution is not established, this page shows the abstract only. Follow the links below for the full text.
Abstract
We report a single-cell bisulfite sequencing (scBS-seq) method that can be used to accurately measure DNA methylation at up to 48.4% of CpG sites. Embryonic stem cells grown in serum or in 2i medium displayed epigenetic heterogeneity, with '2i-like' cells present in serum culture. Integration of 12 individual mouse oocyte datasets largely recapitulated the whole DNA methylome, which makes scBS-seq a versatile tool to explore DNA methylation in rare cells and heterogeneous populations.
Medical subject headings
- Epigenesis, Genetic
- Genome
- Sulfites