High-resolution reconstruction of the beating zebrafish heart.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 25042787.
- Also identified by DOI 10.1038/nmeth.3037.
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Abstract
The heart's continuous motion makes it difficult to capture high-resolution images of this organ in vivo. We developed tools based on high-speed selective plane illumination microscopy (SPIM), offering pristine views into the beating zebrafish heart. We captured three-dimensional cardiac dynamics with postacquisition synchronization of multiview movie stacks, obtained static high-resolution reconstructions by briefly stopping the heart with optogenetics and resolved nonperiodic phenomena by high-speed volume scanning with a liquid lens.
Medical subject headings
- Cell Tracking
- Image Enhancement
- Image Interpretation, Computer-Assisted
- Imaging, Three-Dimensional
- Microscopy, Video
- Myocytes, Cardiac
- Zebrafish