Experimental proof of concept of nanoparticle-assisted STED.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 25053232.
- Also identified by DOI 10.1021/nl5014103.
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Abstract
We imaged core-shell nanoparticles, consisting of a dye-doped silica core covered with a layer of gold, with a stimulated emission depletion, fluorescence lifetime imaging (STED-FLIM) microscope. Because of the field enhancement provided by the localized surface plasmon resonance of the gold shell, we demonstrate a reduction of the STED depletion power required to obtain resolution improvement by a factor of 4. This validates the concept of nanoparticle-assisted STED (NP-STED), where hybrid dye-plasmonic nanoparticles are used as labels for STED in order to decrease the depletion powers required for subwavelength imaging.
Medical subject headings
- Fluorescent Dyes
- Gold
- Nanoshells