On-demand optical immobilization of Caenorhabditis elegans for high-resolution imaging and microinjection.

Hwang, Hyundoo; Krajniak, Jan; Matsunaga, Yohei; Benian, Guy M; Lu, Hang · Lab Chip · 2014

basic_science · Level V

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Abstract

This paper describes a novel selective immobilization technique based on optical control of the sol-gel transition of thermoreversible Pluronic gel, which provides a simple, versatile, and biocompatible approach for high-resolution imaging and microinjection of Caenorhabditis elegans.

Medical subject headings