A portable lipid bilayer system for environmental sensing with a transmembrane protein.
basic_science · Level V
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- Record sourced from PubMed, PMID 25072468.
- Also identified by DOI 10.1371/journal.pone.0102427 and PMC identifier 4114450.
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Abstract
This paper describes a portable measurement system for current signals of an ion channel that is composed of a planar lipid bilayer. A stable and reproducible lipid bilayer is formed in outdoor environments by using a droplet contact method with a micropipette. Using this system, we demonstrated that the single-channel recording of a transmembrane protein (alpha-hemolysin) was achieved in the field at a high-altitude (∼3623 m). This system would be broadly applicable for obtaining environmental measurements using membrane proteins as a highly sensitive sensor.
Medical subject headings
- Biosensing Techniques
- Environmental Monitoring
- Lipid Bilayers
- Membrane Proteins