Rapid and high-throughput formation of 3D embryoid bodies in hydrogels using the dielectrophoresis technique.
basic_science · Level V
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- Record sourced from PubMed, PMID 25082412.
- Also identified by DOI 10.1039/c4lc00479e.
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Abstract
In this manuscript, we demonstrate the rapid formation of three-dimensional (3D) embryonic stem cell (ESC) aggregates with controllable sizes and shapes in hydrogels using dielectrophoresis (DEP). The ESCs encapsulated within a methacrylated gelatin (GelMA) prepolymer were introduced into a DEP device and, upon applying an electric field and crosslinking of the GelMA hydrogel, formed 3D ESC aggregates. Embryoid bodies (EBs) fabricated using this method showed high cellular viability and pluripotency. The proposed technique enables production of EBs on a large scale and in a high-throughput manner for potential cell therapy and tissue regeneration applications.
Medical subject headings
- Cell Culture Techniques
- Electrophoresis
- Embryoid Bodies
- High-Throughput Screening Assays
- Hydrogels