High-resolution structure determination by continuous-rotation data collection in MicroED.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 25086503.
- Also identified by DOI 10.1038/nmeth.3043 and PMC identifier 4149488.
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Abstract
MicroED uses very small three-dimensional protein crystals and electron diffraction for structure determination. We present an improved data collection protocol for MicroED called 'continuous rotation'. Microcrystals are continuously rotated during data collection, yielding more accurate data. The method enables data processing with the crystallographic software tool MOSFLM, which resulted in improved resolution for the model protein lysozyme. These improvements are paving the way for the broad implementation and application of MicroED in structural biology.
Medical subject headings
- Imaging, Three-Dimensional
- Information Storage and Retrieval
- Lasers
- Microscopy, Video
- Proteins
- X-Ray Diffraction