Efficient CRISPR-Cas9-mediated genome editing in Plasmodium falciparum.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 25108687.
- Also identified by DOI 10.1038/nmeth.3063 and PMC identifier 4199390.
- No licence information is recorded for this record.
- Because redistribution is not established, this page shows the abstract only. Follow the links below for the full text.
Abstract
Malaria is a major cause of global morbidity and mortality, and new strategies for treating and preventing this disease are needed. Here we show that the Streptococcus pyogenes Cas9 DNA endonuclease and single guide RNAs (sgRNAs) produced using T7 RNA polymerase (T7 RNAP) efficiently edit the Plasmodium falciparum genome. Targeting the genes encoding native knob-associated histidine-rich protein (kahrp) and erythrocyte binding antigen 175 (eba-175), we achieved high (≥ 50-100%) gene disruption frequencies within the usual time frame for generating transgenic parasites.
Medical subject headings
- Animals, Genetically Modified
- CRISPR-Cas Systems
- Genetic Engineering
- Genome
- Plasmodium falciparum
- RNA Editing