Rapid detection of shrimp white spot syndrome virus by real time, isothermal recombinase polymerase amplification assay.
basic_science · Level V
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- Record sourced from PubMed, PMID 25121957.
- Also identified by DOI 10.1371/journal.pone.0104667 and PMC identifier 4133268.
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Abstract
White spot syndrome virus (WSSV) causes large economic losses to the shrimp aquaculture industry, and thus far there are no efficient therapeutic treatments available against this lethal virus. In this study, we present the development of a novel real time isothermal recombinase polymerase amplification (RPA) assay for WSSV detection on a small ESEQuant Tube Scanner device. The RPA sensitivity, specificity and rapidity were evaluated by using a plasmid standard as well as viral and shrimp genomic DNAs. Compared with qPCR, the RPA assay revealed more satisfactory performance. It reached a detection limit up to 10 molecules in 95% of cases as determined by probit analysis of 8 independent experiments within 6.41 ± 0.17 min at 39 °C. Consequently, this rapid RPA method has great application potential for field use or point of care diagnostics.
Medical subject headings
- Real-Time Polymerase Chain Reaction
- White spot syndrome virus 1