Multiplexed aberration measurement for deep tissue imaging in vivo.
basic_science · Level V
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- Record sourced from PubMed, PMID 25128976.
- Also identified by DOI 10.1038/nmeth.3068 and PMC identifier 4180771.
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Abstract
We describe an adaptive optics method that modulates the intensity or phase of light rays at multiple pupil segments in parallel to determine the sample-induced aberration. Applicable to fluorescent protein-labeled structures of arbitrary complexity, it allowed us to obtain diffraction-limited resolution in various samples in vivo. For the strongly scattering mouse brain, a single aberration correction improved structural and functional imaging of fine neuronal processes over a large imaging volume.
Medical subject headings
- Brain
- Light
- Neuroimaging
- Optics and Photonics