Spherical nucleic acids as a divergent platform for synthesizing RNA-nanoparticle conjugates through enzymatic ligation.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 25144723.
- Also identified by DOI 10.1021/nn503601s and PMC identifier 4174098.
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Abstract
Herein, we describe a rapid, divergent method for using spherical nucleic acids (SNAs) as a universal platform for attaching RNA to DNA-modified nanoparticles using enzyme-mediated techniques. This approach provides a sequence-specific method for the covalent attachment of one or more in vitro transcribed RNAs to a universal SNA scaffold, regardless of RNA sequence. The RNA-nanoparticle constructs are shown to effectively knock down two different gene targets using a single, dual-ligated nanoparticle construct.
Medical subject headings
- DNA Ligases
- Nanoparticles
- Nanotechnology
- Nucleic Acid Conformation
- RNA