Flow-induced immobilization of glucose oxidase in nonionic micellar nanogels for glucose sensing.
basic_science · Level V
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- Record sourced from PubMed, PMID 25144867.
- Also identified by DOI 10.1039/c4lc00610k.
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Abstract
A simple microfluidic platform was utilized to immobilize glucose oxidase (GOx) in a nonionic micellar scaffold. The immobilization of GOx was verified by using a combination of cryogenic electron microscopy (cryo-EM), scanning electron microscopy (SEM), and ultraviolet spectroscopy (UV) techniques. Chronoamperometric measurements were conducted on nanogel-GOx scaffolds under different glucose concentrations, exhibiting linear amperometric responses. Without impacting the lifetime and denaturation of GOx, the nonionic nanogel provides a favorable microenvironment for GOx in biological media. This flow-induced immobilization method in a nonionic nanogel host matrix opens up new pathways for designing a simple, fast, biocompatible, and cost-effective process to immobilize biomolecules that are averse to ionic environments.
Medical subject headings
- Biosensing Techniques
- Enzymes, Immobilized
- Glucose
- Glucose Oxidase
- Micelles
- Microfluidic Analytical Techniques
- Polyethylene Glycols
- Polyethyleneimine