Surface presentation of functional peptides in solution determines cell internalization efficiency of TAT conjugated nanoparticles.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 25157643.
- Also identified by DOI 10.1021/nl5021848 and PMC identifier 5408925.
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Abstract
Functionalizing nanoparticles with cell-penetrating peptides is a popular choice for cellular delivery. We investigated the effects of TAT peptide concentration and arrangement in solution on functionalized nanoparticles' efficacy for membrane permeation. We found that cell internalization correlates with the positive charge distribution achieved prior to nanoparticle encountering interactions with membrane. We identified a combination of solution based properties required to maximize the internalization efficacy of TAT-functionalized nanoparticles.
Medical subject headings
- Gold
- Lipid Bilayers
- Nanoparticles
- Peptides
- tat Gene Products, Human Immunodeficiency Virus