Intravitreal vascular endothelial growth factor concentration and axial length.

Abstract

To examine the intravitreal concentration of vascular endothelial growth factor (VEGF) in dependence of the axial length in eyes without intraocular neovascularization. The concentrations of VEGF in vitreous samples and blood samples of patients undergoing pars plana vitrectomy for treatment of idiopathic macular holes or epiretinal membranes were examined using enzyme-linked immunosorbent assay. Axial length was determined by biometry. The study included 34 patients with macular holes (n = 21) or epiretinal membranes (n = 13) with a mean age of 62.4 ± 10.5 years (range: 35-76 years) and a mean axial length of 24.1 ± 1.8 mm (range: 21.0-29.1 mm). The intravitreal VEGF concentration (mean: 71.0 ± 63.2 pg/mL) was significantly (P < 0.001) lower than the VEGF concentration in the blood (830 ± 585 pg/mL). Both parameters were significantly associated with each other (P = 0.04; correlation coefficient r = 0.35). The intravitreal VEGF concentration was not significantly associated with gender (P = 0.25) or age (P = 0.48). A higher intravitreal VEGF concentration was significantly associated with a shorter axial length (P = 0.025; r = -0.39) or a higher hyperopic refractive error (P = 0.04; r = 0.35). Neither plasma concentration of VEGF nor the ratio of plasma VEGF concentrations to intravitreal VEGF concentration was significantly associated with axial length (P = 0.89 and P = 0.22, respectively) or with refractive error (P = 0.89 and P = 0.26, respectively). Intravitreal VEGF concentration decreased with increasing axial length. It suggests a diluting effect in eyes with larger intraocular volume and/or a faster turnover rate of VEGF in axially myopic eyes with vitreous liquefaction.