A critique of methods for temperature imaging in single cells.

Baffou, Guillaume; Rigneault, Hervé; Marguet, Didier; Jullien, Ludovic · Nat Methods · 2014

basic_science · Level V

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Abstract

We argue that standard thermodynamic considerations and scaling laws show that a single cell cannot substantially raise its temperature by endogenous thermogenesis. This statement seriously questions the interpretations of recent work reporting temperature heterogeneities measured in single living cells.

Medical subject headings