Presentation of fibronectin fragments using affinity protein interactions for enhanced retention and function.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 25173838.
- Also identified by DOI 10.1016/j.actbio.2014.08.026.
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Abstract
We present a protein immobilization system, based on the Src Homology 3 (SH3) affinity domain, allowing for a transient interaction between a fibronectin ligand and a biomaterial surface. This strategy leads to enhanced retention of the fibronectin fragment over adsorbed fibronectin, and increased cellular proliferation and motility over either covalently immobilized or adsorbed fibronectin. The results indicate that intermediate affinity protein immobilization could provide benefits for tissue engineering beyond the traditional immobilization techniques, adsorption or covalent attachment.
Medical subject headings
- Adaptor Proteins, Signal Transducing
- Cell Movement
- Cell Proliferation
- Coated Materials, Biocompatible
- Fibronectins
- Phosphoproteins
- Tissue Engineering
- src Homology Domains