A sentinel protein assay for simultaneously quantifying cellular processes.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 25194849.
- Also identified by DOI 10.1038/nmeth.3101.
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Abstract
We describe a proteomic screening approach based on the concept of sentinel proteins, biological markers whose change in abundance characterizes the activation state of a given cellular process. Our sentinel assay simultaneously probed 188 biological processes in Saccharomyces cerevisiae exposed to a set of environmental perturbations. The approach can be applied to analyze responses to large sets of uncharacterized perturbations in high throughput.
Medical subject headings
- Computational Biology
- Proteomics
- Saccharomyces cerevisiae
- Saccharomyces cerevisiae Proteins
- Systems Biology