Reversible positioning of single molecules inside zero-mode waveguides.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 25209321.
- Also identified by DOI 10.1021/nl503134x and PMC identifier 4189617.
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Abstract
We have developed a hybrid nanopore/zero-mode waveguide device for single-molecule fluorescence and DNA sequencing applications. The device is a freestanding solid-state membrane with sub-5 nm nanopores that reversibly delivers individual biomolecules to the base of 70 nm diameter waveguides for interrogation. Rapid and reversible molecular loading is achieved by controlling the voltage across the device. Using this device we demonstrate protein and DNA loading with efficiency that is orders of magnitude higher than diffusion-based molecular loading.
Medical subject headings
- Nanopores
- Nanotechnology
- Optical Imaging
- Sequence Analysis, DNA