Using amino-labeled nucleotide probes for simultaneous single molecule RNA-DNA FISH.
basic_science · Level V
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- Record sourced from PubMed, PMID 25226542.
- Also identified by DOI 10.1371/journal.pone.0107425 and PMC identifier PMC4062100.
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Abstract
Using amino-labeled oligonucleotide probes, we established a simple, robust and low-noise method for simultaneous detection of RNA and DNA by fluorescence in situ hybridization, a highly useful tool to study the large pool of long non-coding RNAs being identified in the current research. With probes either chemically or biologically synthesized, we demonstrate that the method can be applied to study a wide range of RNA and DNA targets at the single-cell and single-molecule level in cellular contexts.