Nanoparticle tension probes patterned at the nanoscale: impact of integrin clustering on force transmission.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 25238229.
- Also identified by DOI 10.1021/nl501912g and PMC identifier 4189618.
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Abstract
Herein we aimed to understand how nanoscale clustering of RGD ligands alters the mechano-regulation of their integrin receptors. We combined molecular tension fluorescence microscopy with block copolymer micelle nanolithography to fabricate substrates with arrays of precisely spaced probes that can generate a 10-fold fluorescence response to pN-forces. We found that the mechanism of sensing ligand spacing is force-mediated. This strategy is broadly applicable to investigating receptor clustering and its role in mechanotransduction pathways.
Medical subject headings
- Fibronectins
- Gold
- Integrins
- Mechanotransduction, Cellular
- Metal Nanoparticles
- Oligopeptides