Digital microfluidic processing of mammalian embryos for vitrification.
basic_science · Level V
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- Record sourced from PubMed, PMID 25250666.
- Also identified by DOI 10.1371/journal.pone.0108128 and PMC identifier PMC2881200.
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Abstract
Cryopreservation is a key technology in biology and clinical practice. This paper presents a digital microfluidic device that automates sample preparation for mammalian embryo vitrification. Individual micro droplets manipulated on the microfluidic device were used as micro-vessels to transport a single mouse embryo through a complete vitrification procedure. Advantages of this approach, compared to manual operation and channel-based microfluidic vitrification, include automated operation, cryoprotectant concentration gradient generation, and feasibility of loading and retrieval of embryos.