ABRO1 suppresses tumourigenesis and regulates the DNA damage response by stabilizing p53.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 25283148.
- Also identified by DOI 10.1038/ncomms6059 and PMC identifier 4205886.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Abraxas brother 1 (ABRO1) has been reported to be a component of the BRISC complex, a multiprotein complex that specifically cleaves 'Lys-63'-linked ubiquitin. However, current knowledge of the functions of ABRO1 is limited. Here we report that ABRO1 is frequently downregulated in human liver, kidney, breast and thyroid gland tumour tissues. Depletion of ABRO1 in cancer cells reduces p53 levels and enhances clone formation and cellular transformation. Conversely, overexpression of ABRO1 suppresses cell proliferation and tumour formation in a p53-dependent manner. We further show that ABRO1 stabilizes p53 by facilitating the interaction of p53 with USP7. DNA-damage induced accumulation of endogenous ABRO1 as well as translocation of ABRO1 to the nucleus, and the induction of p53 by DNA damage is almost completely attenuated by ABRO1 depletion. Our study shows that ABRO1 is a novel p53 regulator that plays an important role in tumour suppression and the DNA damage response.
Medical subject headings
- DNA Damage
- Gene Expression Profiling
- Nuclear Matrix-Associated Proteins
- Tumor Suppressor Protein p53
- Ubiquitin-Specific Proteases