An improved surface passivation method for single-molecule studies.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 25306544.
- Also identified by DOI 10.1038/nmeth.3143 and PMC identifier 4245390.
- No licence information is recorded for this record.
- Because redistribution is not established, this page shows the abstract only. Follow the links below for the full text.
Abstract
We report a surface passivation method based on dichlorodimethylsilane (DDS)-Tween-20 for in vitro single-molecule studies, which, under the conditions tested here, more efficiently prevented nonspecific binding of biomolecules than the standard poly(ethylene glycol) surface. The DDS-Tween-20 surface was simple and inexpensive to prepare and did not perturb the behavior and activities of tethered biomolecules. It can also be used for single-molecule imaging in the presence of high concentrations of labeled species in solution.
Medical subject headings
- DNA-Directed DNA Polymerase
- Fluorescence Resonance Energy Transfer
- Polyethylene Glycols
- Polysorbates
- Proliferating Cell Nuclear Antigen
- Silanes