A medium throughput device to study the effects of combinations of surface strains and fluid-flow shear stresses on cells.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 25377548.
- Also identified by DOI 10.1039/c4lc01259c.
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Abstract
We report a medium throughput device to study the effects of combinations of two mechanical stimuli - surface strains and fluid flow shear stresses, on cells. The first generation prototype can screen combinations of five strain and five shear stress levels. Computational modeling and empirical measurements were used to determine the generated strains and flows. Uniform equibiaxial strains up to 20% and shear stresses up to 0.3 Pa can be generated. Compatibility of the device with cell culture and end point fixation, staining and imaging is shown using C2C12 mouse myoblast cells.
Medical subject headings
- Cell Culture Techniques