flowDensity: reproducing manual gating of flow cytometry data by automated density-based cell population identification.
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- Record sourced from PubMed, PMID 25378466.
- Also identified by DOI 10.1093/bioinformatics/btu677 and PMC identifier 4325545.
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Abstract
flowDensity facilitates reproducible, high-throughput analysis of flow cytometry data by automating a predefined manual gating approach. The algorithm is based on a sequential bivariate gating approach that generates a set of predefined cell populations. It chooses the best cut-off for individual markers using characteristics of the density distribution. The Supplementary Material is linked to the online version of the manuscript. R source code freely available through BioConductor (http://master.bioconductor.org/packages/devel/bioc/html/flowDensity.html.). Data available from FlowRepository.org (dataset FR-FCM-ZZBW). rbrinkman@bccrc.ca Supplementary data are available at Bioinformatics online.
Medical subject headings
- Algorithms
- Cell Physiological Phenomena
- Computational Biology
- Flow Cytometry
- Software