Grease matrix as a versatile carrier of proteins for serial crystallography.
basic_science · Level V
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- Record sourced from PubMed, PMID 25384243.
- Also identified by DOI 10.1038/nmeth.3172.
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Abstract
Serial femtosecond X-ray crystallography (SFX) has revolutionized atomic-resolution structural investigation by expanding applicability to micrometer-sized protein crystals, even at room temperature, and by enabling dynamics studies. However, reliable crystal-carrying media for SFX are lacking. Here we introduce a grease-matrix carrier for protein microcrystals and obtain the structures of lysozyme, glucose isomerase, thaumatin and fatty acid-binding protein type 3 under ambient conditions at a resolution of or finer than 2 Å.
Medical subject headings
- Crystallography, X-Ray
- Lubricants
- Proteins