An integrated centrifugo-opto-microfluidic platform for arraying, analysis, identification and manipulation of individual cells.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 25407668.
- Also identified by DOI 10.1039/c4lc01002g.
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Abstract
In this work we present a centrifugal microfluidic system enabling highly efficient collective trapping and alignment of particles such as microbeads and cells, their multi-colour fluorescent detection and subsequent manipulation by optical tweezers. We demonstrate array-based capture and imaging followed by "cherry-picking" of individual particles, first for fluorescently labelled polystyrene (PS) beads and then for cells. Different cell lines are discriminated based on intracellular as well as surface-based markers.
Medical subject headings
- Cell Separation
- Microfluidic Analytical Techniques
- Optical Tweezers