CRISPRdirect: software for designing CRISPR/Cas guide RNA with reduced off-target sites.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 25414360.
- Also identified by DOI 10.1093/bioinformatics/btu743 and PMC identifier 4382898.
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Abstract
CRISPRdirect is a simple and functional web server for selecting rational CRISPR/Cas targets from an input sequence. The CRISPR/Cas system is a promising technique for genome engineering which allows target-specific cleavage of genomic DNA guided by Cas9 nuclease in complex with a guide RNA (gRNA), that complementarily binds to a ∼ 20 nt targeted sequence. The target sequence requirements are twofold. First, the 5'-NGG protospacer adjacent motif (PAM) sequence must be located adjacent to the target sequence. Second, the target sequence should be specific within the entire genome in order to avoid off-target editing. CRISPRdirect enables users to easily select rational target sequences with minimized off-target sites by performing exhaustive searches against genomic sequences. The server currently incorporates the genomic sequences of human, mouse, rat, marmoset, pig, chicken, frog, zebrafish, Ciona, fruit fly, silkworm, Caenorhabditis elegans, Arabidopsis, rice, Sorghum and budding yeast. Freely available at http://crispr.dbcls.jp/. Supplementary data are available at Bioinformatics online.
Medical subject headings
- CRISPR-Cas Systems
- Drug Design
- RNA Editing
- RNA, Guide, CRISPR-Cas Systems
- Software