Reduction of photo bleaching and long term archiving of chemically cleared GFP-expressing mouse brains.
basic_science · Level V
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- Record sourced from PubMed, PMID 25463047.
- Also identified by DOI 10.1371/journal.pone.0114149 and PMC identifier PMC2224998.
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Abstract
Tissue clearing allows microscopy of large specimens as whole mouse brains or embryos. However, lipophilic tissue clearing agents as dibenzyl ether limit storage time of GFP-expressing samples to several days and do not prevent them from photobleaching during microscopy. To preserve GFP fluorescence, we developed a transparent solid resin formulation, which maintains the specimens' transparency and provides a constant signal to noise ratio even after hours of continuous laser irradiation. If required, high-power illumination or long exposure times can be applied with virtually no loss in signal quality and samples can be archived for years.