Genetic targeting of chemical indicators in vivo.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 25486061.
- Also identified by DOI 10.1038/nmeth.3207.
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Abstract
Fluorescent protein reporters have become the mainstay for tracing cellular circuitry in vivo but are limited in their versatility. Here we generated Cre-dependent reporter mice expressing the Snap-tag to target synthetic indicators to cells. Snap-tag labeling worked efficiently and selectively in vivo, allowing for both the manipulation of behavior and monitoring of cellular fluorescence from the same reporter.
Medical subject headings
- Fluorescent Dyes
- Gene Knock-In Techniques
- Genes, Reporter
- Integrases
- Recombinant Fusion Proteins