DNA nanoswitches: a quantitative platform for gel-based biomolecular interaction analysis.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 25486062.
- Also identified by DOI 10.1038/nmeth.3209 and PMC identifier 4336243.
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Abstract
We introduce a nanoscale experimental platform that enables kinetic and equilibrium measurements of a wide range of molecular interactions using a gel electrophoresis readout. Programmable, self-assembled DNA nanoswitches serve both as templates for positioning molecules and as sensitive, quantitative reporters of molecular association and dissociation. We demonstrated this low-cost, versatile, 'lab-on-a-molecule' system by characterizing ten different interactions, including a complex four-body interaction with five discernible states.
Medical subject headings
- DNA, Circular
- DNA, Single-Stranded
- Electrophoresis, Polyacrylamide Gel
- Microfluidics
- Nanotechnology
- Proteins