Fast native-SAD phasing for routine macromolecular structure determination.
basic_science · Level V
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- Record sourced from PubMed, PMID 25506719.
- Also identified by DOI 10.1038/nmeth.3211.
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Abstract
We describe a data collection method that uses a single crystal to solve X-ray structures by native SAD (single-wavelength anomalous diffraction). We solved the structures of 11 real-life examples, including a human membrane protein, a protein-DNA complex and a 266-kDa multiprotein-ligand complex, using this method. The data collection strategy is suitable for routine structure determination and can be implemented at most macromolecular crystallography synchrotron beamlines.
Medical subject headings
- DNA-Binding Proteins
- Membrane Proteins
- Multiprotein Complexes
- X-Ray Diffraction