Fixation-resistant photoactivatable fluorescent proteins for CLEM.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 25581799.
- Also identified by DOI 10.1038/nmeth.3225 and PMC identifier 4344411.
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Abstract
Fluorescent proteins facilitate a variety of imaging paradigms in live and fixed samples. However, they lose their fluorescence after heavy fixation, hindering applications such as correlative light and electron microscopy (CLEM). Here we report engineered variants of the photoconvertible Eos fluorescent protein that fluoresce and photoconvert normally in heavily fixed (0.5-1% OsO4), plastic resin-embedded samples, enabling correlative super-resolution fluorescence imaging and high-quality electron microscopy.
Medical subject headings
- Luminescent Proteins
- Microscopy, Electron, Scanning
- Microscopy, Electron, Transmission