Structure and boosting activity of a starch-degrading lytic polysaccharide monooxygenase.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 25608804.
- Also identified by DOI 10.1038/ncomms6961 and PMC identifier 4338556.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Lytic polysaccharide monooxygenases (LPMOs) are recently discovered enzymes that oxidatively deconstruct polysaccharides. LPMOs are fundamental in the effective utilization of these substrates by bacteria and fungi; moreover, the enzymes have significant industrial importance. We report here the activity, spectroscopy and three-dimensional structure of a starch-active LPMO, a representative of the new CAZy AA13 family. We demonstrate that these enzymes generate aldonic acid-terminated malto-oligosaccharides from retrograded starch and boost significantly the conversion of this recalcitrant substrate to maltose by β-amylase. The detailed structure of the enzyme's active site yields insights into the mechanism of action of this important class of enzymes.
Medical subject headings
- Acids
- Maltose
- Mixed Function Oxygenases
- Oligosaccharides
- Polysaccharides