Novel exons and splice variants in the human antibody heavy chain identified by single cell and single molecule sequencing.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 25611855.
- Also identified by DOI 10.1371/journal.pone.0117050 and PMC identifier 4303433.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Antibody heavy chains contain a variable and a constant region. The constant region of the antibody heavy chain is encoded by multiple groups of exons which define the isotype and therefore many functional characteristics of the antibody. We performed both single B cell RNAseq and long read single molecule sequencing of antibody heavy chain transcripts and were able to identify novel exons for IGHA1 and IGHA2 as well as novel isoforms for IGHM antibody heavy chain.
Medical subject headings
- B-Lymphocytes
- Exons
- Immunoglobulin Heavy Chains
- RNA Splicing
- Sequence Analysis, RNA