Registered report: transcriptional amplification in tumor cells with elevated c-Myc.

Blum, David; Hao, Haiping; McCarthy, Michael; Reproducibility Project: Cancer Biology · Elife · 2015

basic_science · Level V

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Abstract

The Lin et al. (2012), published in Cell in 2012. The experiments that will be replicated are those reported in Figures 3E and 3F. In these experiments, elevated levels of c-Myc in the P493-6 cell model of Burkitt's lymphoma results in an increase of the total level of RNA using UV/VIS spectrophotometry (Figure 3E; Lin et al., 2012) and on the mRNA levels/cell for a large set of genes using digital gene expression technology (Figure 3F; Lin et al., 2012). The Reproducibility Project: Cancer Biology is a collaboration between the eLife.

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