Ratiometric biosensors based on dimerization-dependent fluorescent protein exchange.
basic_science · Level V
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- Record sourced from PubMed, PMID 25622108.
- Also identified by DOI 10.1038/nmeth.3261 and PMC identifier 4344385.
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Abstract
We have developed a versatile new class of genetically encoded fluorescent biosensor based on reversible exchange of the heterodimeric partners of green and red dimerization-dependent fluorescent proteins. We demonstrate the use of this strategy to construct both intermolecular and intramolecular ratiometric biosensors for qualitative imaging of caspase activity, Ca(2+) concentration dynamics and other second-messenger signaling activities.
Medical subject headings
- Biosensing Techniques
- Green Fluorescent Proteins
- Luminescent Proteins