Real-time deformability cytometry: on-the-fly cell mechanical phenotyping.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 25643151.
- Also identified by DOI 10.1038/nmeth.3281.
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Abstract
We introduce real-time deformability cytometry (RT-DC) for continuous cell mechanical characterization of large populations (>100,000 cells) with analysis rates greater than 100 cells/s. RT-DC is sensitive to cytoskeletal alterations and can distinguish cell-cycle phases, track stem cell differentiation into distinct lineages and identify cell populations in whole blood by their mechanical fingerprints. This technique adds a new marker-free dimension to flow cytometry with diverse applications in biology, biotechnology and medicine.
Medical subject headings
- Flow Cytometry