Mapping native disulfide bonds at a proteome scale.
basic_science · Level V
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- Record sourced from PubMed, PMID 25664544.
- Also identified by DOI 10.1038/nmeth.3283.
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Abstract
We developed a high-throughput mass spectrometry method, pLink-SS (http://pfind.ict.ac.cn/software/pLink/2014/pLink-SS.html), for precise identification of disulfide-linked peptides. Using pLink-SS, we mapped all native disulfide bonds of a monoclonal antibody and ten standard proteins. We performed disulfide proteome analyses and identified 199 disulfide bonds in Escherichia coli and 568 in proteins secreted by human endothelial cells. We discovered many regulatory disulfide bonds involving catalytic or metal-binding cysteine residues.
Medical subject headings
- Disulfides
- Mass Spectrometry
- Proteome
- Proteomics