TGF-β3-expressing CD4+CD25(-)LAG3+ regulatory T cells control humoral immune responses.

Okamura, Tomohisa; Sumitomo, Shuji; Morita, Kaoru; Iwasaki, Yukiko; Inoue, Mariko; Nakachi, Shinichiro; Komai, Toshihiko; Shoda, Hirofumi et al. · Nat Commun · 2015

basic_science · Level V

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Abstract

Autoantibodies induce various autoimmune diseases, including systemic lupus erythematosus (SLE). We previously described that CD4(+)CD25(-)LAG3(+) regulatory T cells (LAG3(+) Treg) are regulated by Egr2, a zinc-finger transcription factor required for the induction of T-cell anergy. We herein demonstrate that LAG3(+) Treg produce high amounts of TGF-β3 in an Egr2- and Fas-dependent manner. LAG3(+) Treg require TGF-β3 to suppress B-cell responses in a murine model of lupus. Moreover, TGF-β3- and LAG3(+) Treg-mediated suppression requires PD-1 expression on B cells. We also show that TGF-β3-expressing human LAG3(+) Treg suppress antibody production and that SLE patients exhibit decreased frequencies of LAG3(+) Treg. These results clarify the mechanism of B-cell regulation and suggest therapeutic strategies.

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