Multicolor 3D super-resolution imaging by quantum dot stochastic optical reconstruction microscopy.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 25703291.
- Also identified by DOI 10.1021/nn506952g.
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Abstract
We demonstrate multicolor three-dimensional super-resolution imaging with quantum dots (QSTORM). By combining quantum dot asynchronous spectral blueing with stochastic optical reconstruction microscopy and adaptive optics, we achieve three-dimensional imaging with 24 nm lateral and 37 nm axial resolution. By pairing two short-pass filters with two appropriate quantum dots, we are able to image single blueing quantum dots on two channels simultaneously, enabling multicolor imaging with high photon counts.
Medical subject headings
- Imaging, Three-Dimensional
- Microscopy
- Optical Phenomena
- Quantum Dots