De novo protein structure determination from near-atomic-resolution cryo-EM maps.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 25707029.
- Also identified by DOI 10.1038/nmeth.3287 and PMC identifier 4435692.
- No licence information is recorded for this record.
- Because redistribution is not established, this page shows the abstract only. Follow the links below for the full text.
Abstract
We present a de novo model-building approach that combines predicted backbone conformations with side-chain fit to density to accurately assign sequence into density maps. This method yielded accurate models for six of nine experimental maps at 3.3- to 4.8-Å resolution and produced a nearly complete model for an unsolved map containing a 660-residue heterodimeric protein. This method should enable rapid and reliable protein structure determination from near-atomic-resolution cryo-electron microscopy (cryo-EM) maps.
Medical subject headings
- Chemistry, Physical
- Cryoelectron Microscopy
- Models, Molecular
- Proteins