Optimization and technological development strategies of an antimicrobial extract from Achyrocline alata assisted by statistical design.
Where this comes from
- Record sourced from PubMed, PMID 25710523.
- Also identified by DOI 10.1371/journal.pone.0118574 and PMC identifier 4339785.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Achyrocline alata, known as Jateí-ka-há, is traditionally used to treat several health problems, including inflammations and infections. This study aimed to optimize an active extract against Streptococcus mutans, the main bacteria that causes caries. The extract was developed using an accelerated solvent extraction and chemometric calculations. Factorial design and response surface methodologies were used to determine the most important variables, such as active compound selectivity. The standardized extraction recovered 99% of the four main compounds, gnaphaliin, helipyrone, obtusifolin and lepidissipyrone, which represent 44% of the extract. The optimized extract of A. alata has a MIC of 62.5 μg/mL against S. mutans and could be used in mouth care products.
Medical subject headings
- Achyrocline
- Anti-Infective Agents
- Plant Extracts